Notes
A collection of fragments of understanding in the pursuit of deeper questions.
Contents
- Computational Vision
- Introduction to Data Science
- Translational Neuromodeling
- Deep Learning in Artificial & Biological Neuronal Networks
- Introduction to Neuroinformatics
- Intro to Neuroscience
- Systems Neuroscience
- Readings in Neuroinformatics
- Neurobiology of Consciousness
- Econometrics
- Principles of Finance
- Big Data & Databases
- Game Theory
- Intro to Blockchain
- Marketing Analytics
- Financial Accounting
- Fundamentals of Computer Science
- Foundamentals of Information Technology Law
- Statistics
- Computer Programming
- Macroeconomics
- EU Information Law & Data Economy
- Advanced Mathematics & Statistics
- Principles of Management
- Mathematics
- Microeconomics
How To Measure Spiking Activity in a Biological Neuron
How To Measure Spiking Activity in a Biological Neuron? How would one measure a voltage level change of anything? Using a Voltmeter. The only caveat to tis is probe design. Simply placing the tip of the probe in contact with the membrane is usually inefficient (especially at the 1 or 2 micrometer level), thus there are several techniques to properly "clamp" the membrane.
Electrode design is actually quite a hardware challenge for electrical engineers and improvements are being made every year. Alternatively, Neuronal Spiking can be recorded via fluorescence techniques (Voltage Indicators) - Ace1Q-mNeon & Ace2N-mNeon are examples of them.
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GEVIs undergo a conformational change in response to a voltage change which changes their fluorescence levels. Finally, there are Calcium indicators that do exactly the same thing but undergo a conformational shift in response toa. Change in intracellular calcium concentration. Advatanges of Imaging:
- Allows to simultaneously record populations of neurons.
- Allows cell type (gene specific) labelling and imaging.
- Enables repeated (chronic) imaging over many months.